Stem Cells - Activity 2 - Assignment 1

Assignment 1: Student Vocabulary Aid

The following list of terms and corresponding online resources is intended to help you complete the Assignment in Activity 2.

Scientific Terms:	URL for Learning:
fertilization and embryogenesis	• Nova PBS. (2001). “Life’s Greatest Miracle” Part 4 “The First Two Weeks” and Part 5 “The Embryo Takes Shape.” VHS. 20 November. There are 4:58 and 7:47 minute video clips respectively. (http://www.pbs.org/wgbh/nova/miracle/program.html) • Travis, J. (2000). "Mom's eggs execute dad's mitochondria." Science News 157 (1): 5. (http://www.sciencenews.org/articles/20000101/fob3.asp)
oogenesis zona pellicuda cumulus cells	• Purves et al. (2003). Life: The Science of Biology. Sumanasinc.com. "Meiosis." (http://www.sumanasinc.com/webcontent/anisamples/ majorsbiology/meiosis.html) • "Meiosis," and "Female reproductive cycle." (2004). McGraw Hill Online Learning Center, Animations.. Click on the "start Quiz button and then click on the animation link. (http://highered.mcgraw- hill.com/sites/0072351136/student_view0/animation_activities.html) . Browder L. and Iten L (ed.) (1999). "Oogenesis: A collaborative effort." Dynamic Development. Online. (http://www.ucalgary.ca/UofC/eduweb/virtualembryo/oogen.html)
ovarian stimulation/ HCG	• WebMDHealth. "Human chorionic gonadotropic hormone (hCG) " (http://my.webmd.com/hw/being_pregnant/hw42062.asp) • IVF.com. (2003). "Facts about ovarian hyperstimulation syndrome." (http://www.ivf.com/ohss.html)
calcium activation wave/calcium ionophore	• Jaffe L. (2001). "The calcium tsunami thru fertilizing Medaka fish eggs." Developmental Biology Cinema. Society for Developmental Biology. (http://www.sdbonline.org/archive/dbcinema/jaffe/jaffe.html) • Dow J. "Ionophore." The Dictionary of Cell and Molecular Biology. (http://www.mblab.gla.ac.uk/~julian/dict2.cgi?3347)
SCNT	• Dolan DNA Learning Center. "Cloning 101."Cold Spring Harbor Laboratory. (http://www.dnalc.org/stemcells.html) • WNET/Thirteen. 2004. "Innovation: Miracle cell." Online. Jared Lipworth and Erin Chapman. New York. (http://www.pbs.org/wnet/innovation/episode6.html)
parthenogenesise	• Holding, Carol. (2004). "1st mouse by parthenogenesis?" Online. The Scientist Daily News. BioMed Central, April 21. (http://www.biomedcentral.com/news/20040421/01/) • Rincon, P. (2004). "Mice created without fathers." BBC News World Edition April 21. (http://news.bbc.co.uk/2/hi/science/nature/3643847.stm)
teratoma/embryoid bodies	• Horstman, Mark. (2004). “Grow your own teeth.” ABC Science Online. February 17 (http://www.abc.net.au/science/news/health/HealthRepublish_1046144.htm) • CreationEvolutionDesignBlog. (2006. Scientists Find Chickens Retain ancient Ability to Grow Teeth. (http://creationevolutiondesign.blogspot.com/2006/03/scientists-find-chickens-retain.html)
Karyotyping and Mitomycin C	• University of Utah, Genetic Science Learning Center. (2004). "What can our chromosomes tell us?" (http://gslc.genetics.utah.edu/units/disorders/karyotype/) • Warren, D. K. (1996). "Web karyotyping activity. The biology project [Online]" (http://www.biology.arizona.edu/human_bio/activities/karyotyping/karyotyping.html) • A.G. Scientific Inc. "Mitomycin C." (http://www.agscientific.com/Item/M1108.htm)
LIF and gp130	• (2001). "Appendix B, Mouse embryonic cells," in Stem cells: Scientific progress and future research direction. National Institutes of Health. Washington DC. See page 3 Figure B.2 (http://stemcells.nih.gov/info/scireport/)
SCID Mouse	• NIH. (2001). "Autoimmune diseases and the promise of stem cell based therapies, Chapter 6," in Stem cells: Scientific progress and future research directions. National Institutes of Health. Washington DC. See pages 62 and 64. (http://stemcells.nih.gov/info/scireport/)
immunoctyochemistry	• (2001) "Appendix E, Stem cell markers," in Stem cells: Scientific progress and future research directions. . National Institutes of Health. Washington DC. See Figures E.i.1 and E. i.2 (http://stemcells.nih.gov/info/scireport/) • ImmunohistochemistryWorld. (2003). (http://www.ihcworld.com/introduction.htm#intro) • Sumanasinc.com.(2002). "ELISA animation." Perry et al. (http://www.sumanasinc.com/webcontent/ anisamples/molecularbiology/ELISA.html)
pronase	• PROWL. "Pronase." ProteoMetrics and Rockefeller University. (http://prowl.rockefeller.edu/recipes/gproteas/pronase.htm) • The Third Horizon Symposium. (2003) "Signaling scissors: The new perspective on proteases." Palazzo Arzaga, Italy October 23-25. Nature Publishing and Aventis Corp. Click on background and read other articles. (http://www.nature.com/horizon/ proteases/background/prespective.htm)
Oct4	• (2001). "Appendix A, Early development," in Stem cells: Scientific progress and future research directions. National Institutes of Health. Washington DC. (http://stemcells.nih.gov/info/scireport/)
genomic imprinting	(2001). "Appendix A, Early development," in Stem cells: Scientific progress and future research directions. National Institutes of Health. Washington DC. (http://stemcells.nih.gov/info/scireport/) • Genetics Science Learning Center at the Eccles Institute of Human Genetics University of Utah. "What is a stem cell?" (http://gslc.genetics.utah.edu/units/stemcells/whatissc/) • Travis J. (2004). "Code Breakers." Science News 165: 106-107.
gel electrophoresis	• University of Utah, Genetic Science Learning Center. (2004). "Gel electrophoresis." (http://gslc.genetics.utah.edu/units/biotech/gel/) • Purves et al. (2003). Life: The science o biology. Sumanasinc.com. "Gel electrophoresis." (http://www.sumanasinc.com/webcontent/ anisamples/majorsbiology/gelelectrophoresis.html)
STR DNA polymorphism DNA sequencing	• Butler J.M and. Reeder D.J. "STRs 101: Brief introduction to STRs." Short Tandem Repeat DNA Database. (http://www.cstl.nist.gov/biotech/strbase/intro.htm) be sure to view the powerpoint at the top of the page which demonstrates the technology and rationale.
RT-PCR	• The Dolan DNA Learning Center. "PCR animation." Cold Spring Harbor Laboratory. (http://www.dnalc.org/shockwave/pcranwhole.html) • Campbell M. " RT-PCR animation." (http://www.bio.davidson.edu/people/kabernd/seminar/2002/method/lowry/RTPCR.htm)

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